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CD14 antibody [LPSR/2397]

Anti-CD14 antibody [LPSR/2397] used in Western Blot (WB). GTX18025
Anti-CD14 antibody [LPSR/2397] used in IHC (Paraffin sections) (IHC-P). GTX18025
Anti-CD14 antibody [LPSR/2397] used in IHC (Paraffin sections) (IHC-P). GTX18025

Cat. No. GTX18025

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 LPSR/2397

Isotype

 IgG2b

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($529)
See all CD14 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT

Note :

IHC-P
Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human CD14 protein (around aa 25-148) (exact sequence is proprietary)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD14 , CD14 molecule

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Mar 2010]

Database

Research Area

DATA IMAGES

Anti-CD14 antibody [LPSR/2397] used in Western Blot (WB). GTX18025

GTX18025 WB Image

WB analysis of A549 cell lysate using GTX18025 CD14 antibody [LPSR/2397].

Anti-CD14 antibody [LPSR/2397] used in IHC (Paraffin sections) (IHC-P). GTX18025

GTX18025 IHC-P Image

IHC-P analysis of human lymph node tissue using GTX18025 CD14 antibody [LPSR/2397].

Anti-CD14 antibody [LPSR/2397] used in IHC (Paraffin sections) (IHC-P). GTX18025

GTX18025 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX18025 CD14 antibody [LPSR/2397].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529